miscript mirna pcr array human ovarian cancer  (Qiagen)

Journal: OncoTargets and therapy

Article Title: miRNA expression profiling in formalin-fixed paraffin-embedded endometriosis and ovarian cancer samples

doi: 10.2147/OTT.S137107

Figure Lengend Snippet: qRT-PCR validation in endometriosis and ovarian cancer patient cohort for miR-93, miR-141, miR-155, miR-429, miR-200c, miR-205 and miR-492. ROC curve analysis of expression levels of miRNAs for endometriosis and ovarian cancer group. The figure displays AUC for each evaluated miRNA, a parameter that indicates the precision in discriminating the endometrial tissue and ovarian cancer tissue from the normal tissue. * P <0.05; *** P <0.001. Abbreviations: AUC, area under the curve; qRT-PCR, quantitative real-time PCR; ROC, receiver operating characteristic.

Article Snippet: The cDNA was then diluted and used for PCR array based on a SybrGreen protocol and Human Ovarian Cancer miScript miRNA PCR array plate (MIHS-110Z; Qiagen NV, Venlo, the Netherlands) following the protocol recommended by the manufacturer.

Techniques: Quantitative RT-PCR, Expressing, Real-time Polymerase Chain Reaction

Journal: EBioMedicine

Article Title: CCAAT/enhancer binding protein delta (C/EBPδ) demonstrates a dichotomous role in tumour initiation and promotion of epithelial carcinoma

doi: 10.1016/j.ebiom.2019.05.002

Figure Lengend Snippet: C/EBPδ overexpression shows differential expression of epithelial and mesenchymal markers at the protein and mRNA level. a. A quantitative PCR (qPCR) measured the relative mRNA expression levels of C/EBPδ, and EMT/MET markers, including Snail, Slug, Zeb1, Zeb2 in two FTE cell lines overxpresing C/EBPδ verses control cells. b. A western blot analysis of EMT/MET protein markers perfomed on 57-FTE and 19-FTE overexpressing C/EBPδ compared to control cells. c. A qPCR of miRNA was performed on both 57-FTE and 19-FTE cells showing the fold change in various miRNAs in cells overexpressing C/EBPδ compared to control cells. Upregulated miRNA (Red) and downregulated (Green) were identified ( p < 0.05, n = 3). Data are represented as mean +/− SEM and p -values are calculated using Student t -tests, two-tailed, and a 2-way ANOVA. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: RNA was reverse transcribed into cDNA using miScript II RT kit (Qiagen). cDNA was then processed using miScript SYBR Green PCR kit (Qiagen) and was run on miScript miRNA PCR Array Human Ovarian Cancer plates (Qiagen, MIHS-110ZE-4, 384-well plate).

Techniques: Over Expression, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Two Tailed Test